Because of the relatively high price tag, many researchers find it beneficial to screen siRNA sequences using a less expensive preparation method, such as in vitro transcription, and then have the most effective sequence(s) synthesized chemically. Drawbacks include the price and turnaround times (typically 4–12 days depending on synthesis and purification options). One of the major benefits of chemical synthesis is the large yield of high purity siRNA. Ambion, and several other companies, provide high quality, chemically synthesized siRNAs on a custom basis. *Depends on purification/deprotection options selected and format (e.g., annealed and ready to transfect versus single strands supplied lyophilized)Īlthough more expensive than any of the other methods, the production of chemically synthesized siRNAs requires almost no effort by the researcher. Silencer Express siRNA Expression Cassette Kits Relative cost per gene (not including labor) Monitor transfection efficiency of entire population Selectability (i.e, antibiotic selection) Transcription template (200-800 bp region flanked by T7 promoters)ġ day + transcription template preparation timeĪbility to label siRNA (i.e., for analyzing siRNA uptake or localization by fluorescence microscopy) Using Validated siRNAs in Functional Genomic Assays.Understanding Calculations for siRNA Data.siRNA-Induced mRNA Knockdown and Phenotype.siRNA Screening Validate Thousands of Targets in a Single Week.siRNA Expression Vectors with Selectable Markers.Silencer® siRNA Starter Kit | New User's Kit for Gene Silencing.Silencer® siRNA Screening Control Panel | Effective Controls for RNAi Screening Experiments.Silencer® siRNA Libraries | siRNA Libraries Targeting Important Functional Gene Classes.Setting up Successful siRNA Library Screens.Reproducibly Deliver siRNAs into Cultured Cells.Reduced siRNA Concentrations Lead to Fewer Off-Target Effects.Recommendations for Successful siRNA Library Screens.Quickly Assess siRNA Delivery and Cell Viability in the Same Assay.Next Generation siRNAs to Make Your Silencing Roar.High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells.Tips from the Bench: Get Control of Your siRNA Experiments.Fluorescently Label Your siRNA to Track it in Live Cells.Fast and Accurate Confirmation of Gene Silencing | Silencer® siRNAs & TaqMan® Gene Expression Assays.Experimental Variability and Replicates in siRNA Experiments.Enhanced siRNA Delivery and Long-term Gene Silencing.Duration of siRNA Induced Silencing: Your Questions Answered.Delineating the Role of Survivin in Oncogenesis: An siRNA Study.Controlling Variability in Cell Assays When Designing RNAi Experiments.Control Your siRNA Research | Proven siRNA Controls and Matched Primary Antibodies.Cells-to-cDNA™ II Applications | Quantitation of siRNA Target Gene Expression.Assessing Gene Function with siRNA Libraries.
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